Composite

Part:BBa_K3799007:Design

Designed by: Lakshmi Prakash   Group: iGEM21_IISER_Kolkata   (2021-10-01)


Unidirectional model of AIP-1 sensor of S aureus regulating GFP intensity using P2 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2953


Design Notes

The DNA fragment was obtained from iGEM kit and further amplified using Biobrick prefix and suffix primers


Source

Sequence for this gene is obtained from the genomic DNA sequence of S.aureus.


References